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E3158 Sigma-Aldrich

Anti-ERG (171-185) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ERG Isoform 5 of Transcriptional regulator ERG, Anti-Erg-3, Anti-p55

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Transcription Factors, EA - G999,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:2,000
species reactivity   human
mol wt   antigen ~54 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P11308
Gene Information   human ... ERG(2078)

Description

Immunogen

synthetic peptide corresponding to amino acids 171-185 of human ERG

Application

Anti-ERG (171-185) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

ERG (ETS-related gene) is an oncogene encoding for a protein that is mutated in cancer. It belongs to the Ets family of transcription factors and is critically important for maintenance of hematopoietic stem cell population. Majority of human prostate cancers is caused due to overexpression of ERG. ERG overexpression is found among intermediate and high-risk tumors. ERG expression has potential to act as biomarkers and therapeutic targets for prostate tumors. It is also useful in inhibiting cell differentiation and activating cell growth.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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