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  • E4013 - Monoclonal Anti-Elastin antibody produced in mouse

E4013 Sigma-Aldrich

Monoclonal Anti-Elastin antibody produced in mouse

clone BA-4, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
conjugate   unconjugated
clone   BA-4, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue
  microarray: suitable
species reactivity   goat, feline, guinea pig, human, sheep, bovine, pig, canine
mol wt   antigen 67.5 kDa
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P15502
Gene Information   human ... ELN(2006)


General description

Monoclonal Anti-Elastin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Elastin is an important polymeric protein of connective tissue. It is synthesized and secreted as a soluble single-chain protein (tropoelastin) that undergoes numerous post-ribosomal modifications prior to the organization of the elastic fiber in the extracellular space.


The antibody binds to insoluble elastin, α-elastin, tropoelastin (the soluble non-cross linked precursor of elastin) and to peptide fragments generated by proteolytic digestion of insoluble elastin. In tropoelastin preparations, three immunoreactive bands have been observed at 67.5 kDa, 65 kDa and 62 kDa corresponding to the three elastin isoforms. The antibody is specific for an epitope composed of the repeated sequence in elastin: Val-Gly-Val-Ala-Pro-Gly. This epitope has been shown to be a chemoattractant for fibroblasts and monocytes.


bovine α-elastin.


Monoclonal Anti-Elastin antibody produced in mouse has been used in:
• immunohistochemistry
• immunostaining
• immunoblotting


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function.

Elastin undergoes changes of morphology with aging and in many disease states. For example, abnormal accumulation of connective tissue in blood vessels contributes to alterations in vascular physiology associated with diseases such as hypertension and atherosclerosis. Several heritable diseases such as Marfan syndrome, pseudoxanthoma elasticum and the Buschke-Ollendorf syndrome may result from molecular defects of elastin.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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