• USA Home
  • E4127 - Epidermal Growth Factor from murine submaxillary gland

EMAIL THIS PAGE TO A FRIEND
E4127 Sigma-Aldrich

Epidermal Growth Factor from murine submaxillary gland

EGF, suitable for cell culture

Synonym: EGF, Epidermal cell growth factor

Purchase

Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents,
biological source   mouse submaxillary glands
form   lyophilized powder
potency   0.1-10 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   6 kDa
packaging   pkg of 0.1 and 5X0.1 mg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤50 EU/vial (LAL test)
color   white to off-white
solubility   water: soluble, clear, colorless (1 vial is reconstituted with 0.5 mL of 0.85% NaCl)
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   mouse ... Egf(13645)

Description

Application

Epidermal Growth Factor (EGF) from murine submaxillary gland has been used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone. The working concentration for EGF is generally 0.1-10 ng/mL.

Packaging

0.1 mg in glass bottle

Biochem/physiol Actions

EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells, rabbit chondrocytes. EGF also plays a biological role in inhibition of gastric acid secretion, support of growth during fetal development and neuromodulation in the central nervous system. Stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins are all cellular metabolic effects of EGF.

Components

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. Human EGF is an identical molecule to ß-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-a, and both exert their actions through EGF receptors.

Caution

This product should be stored at 2-8°C. After reconstitution, it can be stored at 2-8°C for two weeks, or frozen in aliquots at -20°C for longer periods.

Preparation Note

This product was lyophilized from a solution in 1 mL of 5 mM ammonium acetate at pH 6.5. It should be reconstituted by adding the contents of the vial to a solution containing 0.1-1.0% BSA or 1-10% serum in buffered saline or tissue culture medium.

Analysis Note

The biological activity of this product is measured by an ability to stimulate the EGF-responsive mouse BALB/MK cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
KB4518000

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Certificate of Origin

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
Keywords: Apoptosis, Cancer, Cell proliferation, Degradations, Diseases, Electron spin resonance, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Transcription, Transduction

Epidermal Growth Factors (EGF) and NRG Family

The epidermal growth factor (EGF) family of growth factors is comprised of the normal mammalian gene products of EGF, amphiregulin, betacellulin, heparin-binding EGF (HB-EGF), and transforming growth...
Jennifer Fries
BioFiles 2009, 4.5, 5.
Keywords: Cancer, Growth factors, PAGE, Type

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Add to Cart

I1882 Insulin from bovine pancreas, γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder
T5391 apo-Transferrin human, γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%
P7527 Prostaglandin E1, γ-irradiated, powder, BioXtra, suitable for cell culture

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?