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E4150 Sigma-Aldrich

Monoclonal Anti-Eotaxin antibody produced in rat

clone 42285.111, purified immunoglobulin, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   42285.111, monoclonal
biological source   rat
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  neutralization: suitable
  western blot: 1-2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
UniProt accession no.   P48298
Gene Information   mouse ... Ccl11(20292)

Description

General description

Accumulation of eosinophils is the hallmark of several medical conditions such as infections and systemic diseases. A number of chemokines stimulate the proliferation, migration and accumulation of eosinophils at the site of inflammation. Eotaxin is chemoattractant that selectively acts on eosinophils and has minimal effect on other leukocytes. It is secreted in response to TNF-α, IL-5 or IL-3. The effect of Eotaxin is mediated by specific eotaxin receptor that induces activation of Gi proteins, MAPK signaling and involves changes in intracellular calcium levels and cytoskeletal reorganization. Eotaxin receptor is present on eosinophils, basophils and T-helper cells. Eotaxin coordinates the accumulation of these cells in response to allergic inflammation and homing of eosinophils to gastrointestinal tract and mucosal tissues
Monoclonal Anti-Eotaxin recognizes mouse eotaxin. This antibody exhibits less than 10 % cross-reactivity with recombinant human eotaxin, recombinant mouse MARC, and recombinant human 6Ckine.

Specificity

Detects mouse CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CCL11/Eotaxin, recombinant mouse CCL7,and rhCCL21 is observed.

Immunogen

recombinant mouse eotaxin.

Application

Anti-eotaxin antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody was used at 1.5 μg/ml concentration to detect eotaxin in metacestode tissue extracts from mice by immunoblotting. For ELISA, a working antibody concentration of 0.5-1.0 μg/ml is recommended. The antibody is suitable for neutralization reactions (ND50 is 10-30 μg/ml).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein G.

Analysis Note

The antibody neutralizes the biological activity of recombinant mouse eotaxin and also human eotaxin.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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