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E4788 Sigma

Plant RNA/DNA Purification Kit

sufficient for 50 purifications

Purchase

Description

Application

For simultaneous isolation of total RNA and DNA from the same plant sample.

Purified RNA and DNA are of the highest quality and can be used in a number of downstream applications including:
• Real-time PCR
• Southern blotting
• SNP analysis
• Sequencing
• AFLP
• RFLP
• RAPD
• Microsatellite analysis
• qRT-PCR
• Primer extension
• Expression array assays
• Next Generation Sequencing

Other Notes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Features and Benefits

• Total RNA and genomic DNA are column purified simultaneously in under 30 minutes.
• Reduces variability as RNA and DNA are isolated from a single plant sample.
• Isolate nucleic acids from small plant samples.
• Isolates total RNA including microRNAs.
• No phenol/chloroform extraction or alcohol precipitation.
• Isolate DNA-free plant RNA or RNA-free plant DNA

General description

Norgen Biotek′s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under 30 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9

Documents

Certificate of Analysis

Protocols & Articles

Articles

A Novel Technology to Extract Nucleic Acids

Preparation of high quality nucleic acids from environmental samples is critical to allow for the use of the purified DNA and RNA in downstream research and diagnostic applications. However, preparat...
Won-Sik Kim1, and Yousef Haj-Ahmad1,2
(1)Norgen Biotek Corp., Thorold, ON, Canada, (2)Brock University. St. Catharines, ON, Canada
Keywords: Amplification, Diagnostic, Environmental, Genetic, Polymerase chain reaction, Purification

Compatibility of DNA and RNA Extraction Methods for Challenging Plant Species

Frequent failure of downstream applications with DNA and RNA is often related to poor sample preparation. In particular, it is often difficult to extract DNA and RNA from samples that contain high le...
Eric Sonke3, Nezar Rghei1, Yousef Haj-Ahmad1,2 and Won-Sik Kim1
(1) Norgen Biotek Corp. 3430 Schmon Parkway, Thorold, Ontario, Canada, L2V 4Y6, (2) Brock University, 500 Glenridge Avenue, St. Catharines, ON, Canada, L2S 3A1, (3) Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, Western University London, ON, Canada.
Keywords: Amplification, Polymerase chain reaction, Sample preparations, Sequencing

Plant RNA/DNA Purification Kit FAQ

From our library of Articles, Sigma-Aldrich presents Plant RNA/DNA Purification Kit FAQ
Keywords: Cell disruption, Centrifugation, Homogenization

Protocols

Plant RNA/DNA Purification Kit

Norgen’s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants. The total RNA and DNA (including...
Keywords: Cell disruption, Centrifugation, Chromatography, Column chromatography, DNA purification, Detergents, Diagnostic, Gene expression, Homogenization, Molecular biology, Polymerase chain reaction, Purification, RNA purification, Separation, Sequencing, Transcription

Peer-Reviewed Papers
15

References

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Add to Cart

STRN10 Spectrum Plant Total RNA Kit, sufficient for 10 purifications
STRN50 Spectrum Plant Total RNA Kit, sufficient for 50 purifications
STRN250 Spectrum Plant Total RNA Kit, sufficient for 250 purifications

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