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E5038 Sigma

Plant/Fungi DNA Isolation Kit Green Alternative

sufficient for 50 purifications

Purchase

Description

Other Notes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

Norgen′s Plant/Fungi DNA Isolation Kit purifies high quality total DNA that can be used in a number of downstream applications, including:
• qPCR
• Southern blotting
• SNP analysis
• Sequencing

Plant/Fungi DNA Isolation Kit has been used for rapid isolation of total DNA from plants and fungi.

General description

Norgen Biotek′s Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and filamentous fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

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Protocols & Articles

Articles

Detection of Genomic DNA from Processed Plant Oils and Wheat Flour

Food processing practices are common in today’s agricultural and food industries, and are often harsh on the genetic information of plants and plant products. Commonly produced oils such as olive oil...
E. Sonke, BMSc1, W. Kim, PhD1, and Y. Haj-Ahmad, Ph.D1,2
(1)Norgen Biotek Corporation, Thorold, Ontario, Canada, (2)Centre for Biotechnology. Brock University, St. Catharines, Ontario, Canada
Keywords: Agriculture, Amplification, Cell disruption, Electrophoresis, Gel electrophoresis, Genetic, Industries, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Plant/Fungi DNA Isolation Kit FAQ

From our library of Articles, Sigma-Aldrich presents Plant/Fungi DNA Isolation Kit FAQ
Keywords: Cell disruption, Nucleic acid annealing, Polymerase chain reaction

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Plant/Fungi DNA Isolation Kit

Norgen’s Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species. Furthermore, the kit also provides a conve...
Keywords: Cell disruption, Centrifugation, Chromatography, Column chromatography, DNA purification, Diagnostic, Homogenization, Nucleic acid annealing, Polymerase chain reaction, Purification, RNA purification, Sequencing

Peer-Reviewed Papers
15

References

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E4913 Plant/Fungi RNA Purification Kit, sufficient for 50 purifications

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