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E5134 Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

  • CAS Number 6381-92-6

  • Empirical Formula (Hill Notation) C10H14N2Na2O8 · 2H2O

  • Molecular Weight 372.24

  •  Beilstein/REAXYS Number 3900609

  •  EC Number 205-358-3

  •  PubChem Substance ID 24894605

  •  NACRES NA.31



Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Chelators,
Quality Level   300
grade   for electrophoresis
  for molecular biology
assay   99.0-101.0% (titration)
form   powder
application(s)   electrophoresis: suitable
impurities   ≤0.005% Insolubles
  ≤0.1% Nitrilotriacetic acid (NTA)
mp   248 °C (dec.) (lit.)
solubility   3  M NaOH: 1.6 g/10 mL
cation traces   Fe: ≤0.005%
  Pb: ≤0.002%
foreign activity   DNase, RNase, NICKase and protease, none detected
storage temp.   room temp
SMILES string   O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI   1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2


General description

Ethylenediaminetetraacetic acid disodium salt dehydrate is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.


Ethylenediaminetetraacetic acid disodium salt dehydrate has been used:
• To prepare TE buffer.
• As an anticoagulant.
• As a constituent of Flow buffer.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.


1 kg in poly bottle

5 kg in poly drum

50, 100, 250, 500 g in poly bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Target organs 
Respiratory Tract
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can solutions of Product E5134, EDTA disodium salt, be autoclaved?
Is Product E5134, EDTA disodium salt, stable in solution?
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.
What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?
The optimal concentration is 1.5 mg per mL of blood.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles


Ethylenediaminetetraacetic acid disodium salt dihydrate Product Information

Molecular Formula: C10H14N2Na2O8 • 2H2O Molecular Weight: 372.2 CAS Number: 6381-92-6 Melting Point: 248 °C pKa: 2.0, 2.7, 6.2, 10.31
Keywords: Anticoagulants, Biological processes, Melting, Molecular biology

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