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E5159 Sigma-Aldrich

Anti-EPHX2 (506-520) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-CEH, Anti-Epoxide hydrolase 2, Anti-SEH

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Properties

Related Categories Alphabetical Index, Antibodies, EO-ER, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:500-1:2,000
species reactivity   human
mol wt   antigen ~63 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P34913
Gene Information   human ... EPHX2(2053)

Description

Immunogen

synthetic peptide corresponding to amino acids 506-520 of human EP of hX2

Application

Anti-EPHX2 (506-520) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Soluble epoxide hydrolase (sEH) is a bifunctional enzyme encoded by the EPHX2 gene in humans. Mammalian sEH converts epoxides to corresponding diols by addition of a water molecule. sEH readily hydrolyzes lipid signaling molecules, including the epoxyeicosatrienoic acids (EETs), epoxidized lipids produced from arachidonic acid by the action of cytochrome p450s. It helps in regulating vascular tone, nociception, angiogenesis and the inflammatory response. sEH plays an important role in the metabolic conversion of the protective eicosanoid 14,15-epoxyeicosatrienoic acid to 14,15-dihydroxyeicosatrienoic acid. Dimerization is required for sEH hydrolase activity and disruption of sEH dimerization may serve as a novel therapeutic strategy for reducing sEH hydrolase activity. Inhibitors of sEH are antihypertensive and renal protective. Its upregulation in proximal tubular cells in chronic proteinuric kidney diseases may mediate proteinuria-induced renal damage. sEH inhibition by increasing renal eicosanoid levels can prevent the progression of chronic proteinuric kidney diseases.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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