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E5389 Sigma

Erythromycin

BioReagent, suitable for cell culture

Synonym: E-Mycin, Erythrocin

  • CAS Number 114-07-8

  • Empirical Formula (Hill Notation) C37H67NO13

  • Molecular Weight 733.93

  •  Beilstein Registry Number 75279

  •  EC Number 204-040-1

  •  MDL number MFCD00084654

  •  PubChem Substance ID 24894612

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Properties

Related Categories A - K, Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z,
InChI Key   ULGZDMOVFRHVEP-RWJQBGPGSA-N
product line   BioReagent
form   powder
potency   ≥850 μg per mg
application(s)   cell culture | mammalian: suitable
impurities   ≤0.1 EU/mg endotoxin
color   white
mp   133 °C
solubility   2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
  ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
Gene Information   human ... ABCB1(5243), CYP3A4(1576), MLNR(2862)
mouse ... Abcb1a(18671), Abcb1b(18669)

Description

Application

Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Biochem/physiol Actions

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.

Caution

This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

Packaging

1, 5 g in poly bottle

Preparation Note

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

General description

Chemical structure: macrolide

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
KF437500
Protocols & Articles

Articles

Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

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Peer-Reviewed Papers
15

References

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