E5389 Sigma


BioReagent, suitable for cell culture

Synonym: E-Mycin, Erythrocin



Related Categories A - K, Antibacterial, Antibiotic powders, Antibiotics, Antibiotics A to Z,
product line   BioReagent
form   powder
potency   ≥850 μg per mg
application(s)   cell culture | mammalian: suitable
impurities   ≤0.1 EU/mg endotoxin
color   white
mp   133 °C
solubility   2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
  ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
Gene Information   human ... ABCB1(5243), CYP3A4(1576), MLNR(2862)
mouse ... Abcb1a(18671), Abcb1b(18669)



Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Biochem/physiol Actions

Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria.


This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.


1, 5 g in poly bottle

Preparation Note

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

General description

Chemical structure: macrolide

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability

Request a Serum Sample

Celebrate Pi Day!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Inhibition of Protein Synthesis by Antibiotics

Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis

Related Content

Antibiotic Selection Guide

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?