• USA Home
  • E5768 - Monoclonal Anti-Eotaxin-2 antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
E5768 Sigma-Aldrich

Monoclonal Anti-Eotaxin-2 antibody produced in mouse

clone 61016.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-MPIF-2

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   61016.11, monoclonal
biological source   mouse
application(s)   capture ELISA: suitable
  neutralization: suitable
  western blot: 1-2 μg/mL
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   O00175
Gene Information   human ... CCL24(6369)

Description

General description

Accumulation of eosinophils is the hallmark of several medical conditions such as infections and systemic diseases. A number of chemokines stimulate the proliferation, migration and accumulation of eosinophils at the site of inflammation. Eotaxin-2 is chemoattractant that selectively acts on eosinophils and has minimal effect on other leukocytes. It is secreted in response to TNF-α, IL-5 or IL-3. The effect of Eotaxin is mediated by specific eotaxin receptor that induces activation of Gi proteins, MAPK signaling and involves changes in intracellular calcium levels and cytoskeletal reorganization. Eotaxin receptor is present on eosinophils, basophils and T-helper cells. Eotaxin coordinates the accumulation of these cells in response to allergic inflammation and homing of eosinophils to gastrointestinal tract and mucosal tissues
Monoclonal Anti-Eotaxin-2 (MPIF-2) recognizes recombinant human eotaxin-2, less than 0.03 % cross-reactivity with recombinant human eotaxin, MIP-1α, and MCP-3.

Immunogen

recombinant human eotaxin-2.

Application

Anti-eotaxin-2 antibody may be used for neutralization assays at a working concentration of 1-5 μg/ml in the presence of 100 ng/ml of human recombinant eotaxin-2. The antibody is suitable for immunoblotting at 1-2 μg/ml concentration. For capture ELISA, a final concentration of 2 μg/ml may be used.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein G

Analysis Note

The antibody neutralizes the biological activity of recombinant human eotaxin-2. It exhibits less than 0.03% cross-reactivity with recombinant human eotaxin, MIP-1α, and MCP-3.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Please Enter a Lot Number

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?