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  • E5900 - Anti-c-erbB-4 antibody, Mouse monoclonal

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E5900 Sigma-Aldrich

Anti-c-erbB-4 antibody, Mouse monoclonal

clone HER4-36, purified from hybridoma cell culture

Synonym: Anti-HER-4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   HER4-36, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): 4-8 μg/test using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4)
  microarray: suitable
species reactivity   human
mol wt   antigen 180-185 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   Q15303
Gene Information   human ... ERBB4(2066)

Description

General description

Monoclonal Anti-c-erbB-4 (erb-b2 receptor tyrosine kinase 4) (HER-4) (mouse IgG1 isotype) is derived from the HER4-36 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-4 from transfected cells. The human ErbB-4 oncogene (also known as HER4) is a 180–185 kDa receptor that binds to a family of isoforms, collectively known as neuregulins. This gene belongs to epidermal growth factor receptor family and is expressed mainly in several breast carcinoma cell lines, heart, brain, cerebellum, pituitary and in normal skeletal muscle.

Immunogen

extracellular domain of human erbB-4 from transfected cells.

Application

Anti-c-erbB-4 antibody, Mouse monoclonal has been used in immunoprecipitation. It has also been used in studies on receptor turnover, phosphorylation, ligand binding and induction of differentiation of cultured breast cancer cells.

Monoclonal anti-c-erbB-4 antibody can be used in immunoprecipitation (4-8 μg/test) using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4). It can also be used in microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The epidermal growth factor receptor (ErbB/HER) family of transmembrane receptor tyrosine kinases significantly participates in defining cell lineage during mesenchyme-epithelial inductive processes and in the neuro-muscular and glia-Schwann cells interactions. It plays a crucial role as a cell surface receptor for neuregulins. It can also bind with erbB -2 to forms heterodimers and can modulate ligand affinity. The catalytic domain is important for the intrinsic tyrosine kinase activity and shows greater degree of homology. Certain ligands and receptors of the family contribute to a relatively virulent phenotype of some human tumors; most notable are carcinomas of secretory epithelia. ErbB-4 also binds to related group of molecules, termed neuregulin 2 (NRG2)(which also binds to erbB-3) and NRG3 (which binds exclusively to erbB-4). Two other ligands, betacellulin and the heparin-binding EGF-like growth factor, bind to both erbB-1 and erbB-4. ErbB-4 forms heterodimers with erbB-2, resulting in the modulation of its ligand affinity.

Safety & Documentation

Safety Information

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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