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E6406 Millipore

EZview Red Glutathione Affinity Gel

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Properties

Related Categories Affinity Chromatography, GST, Glutathione, Glutathione S-transferase (GST), Molecular Biology,
application(s)   immunoprecipitation (IP): suitable
  protein purification: suitable
analyte suitability   proteins (GST)
storage temp.   2-8°C

Description

General description

EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.

Application

The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Packaging

1 mL in poly bottle

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Related Content

EZview™ Red Affinity Gels [VIDEO]

The EZview™ Red Affinity Gels have a vivid red dye that clearly defines the pellet from the supernatant. This enhanced visibility results in less tedious sample processing and greatly reduces the ris...
Keywords: Tagged proteins

Peer-Reviewed Papers
15

References

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