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E6532 Sigma-Aldrich

Anti-eiF4EBP2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-4E-BP2, Anti-Eukaryotic translation initiation factor 4E-binding protein 2, Anti-eiF4E-binding protein 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for RNA Regulation and Translation, Antibodies to Ribosomal Proteins and Translation Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over-expressing human eIF4EBP2
  indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human eIF4EBP2
  western blot: 2-4 μg/mL using lysates of HEK-293T cells over-expressing human eIF4EBP2
species reactivity   human, rat (predicted), mouse (predicted)
mol wt   antigen ~17 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q13542
Gene Information   human ... EIF4EBP2(1979)
mouse ... Eif4ebp2(13688)
rat ... Eif4ebp2(361845)

Description

General description

Eukaryotic translation initiation factor 4E-binding protein 2 (PHASII) is a protein encoded by the EIF4EBP2 gene in humans. Two eukaryotic initiation factor-4E binding proteins (4E-BP1 and 4E-BP2) were found to bind specifically to eIF4E and inhibit its function. 4E-BP2 gene (20kb) exhibits an identical genomic organization to that of 4E-BP1 and is localized to chromosomes 10q21-q22. It is found abundantly in liver and kidney.

Immunogen

synthetic peptide corresponding to amino acids 101-114 of human eIF4EBP2 conjugated to KLH via a cysteine residue. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-eiF4EBP2 antibody produced in rabbit is suitable for the following applications:
• Immunoprecipitation at a concentration of 5-10μg using lysates of HEK-293T cells over-expressing human eIF4EBP2
• Indirect immunofluorescence at a concentration of 2.5-5μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human eIF4EBP2
• Western blotting at a concentration of 2-4μg/mL using lysates of HEK-293T cells over-expressing human eIF4EBP2

Target description

eiF4EBP2 encodes a member of the eukaryotic translation initiation factor 4E binding protein family. The gene products of this family bind eIF4E and inhibit translation initiation. However, insulin and other growth factors can release this inhibition via

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The expression of 4E-BP2 acts as a specific repressor of eIF4E function. Its expression is high in double-positive (DP) cells and decreases during maturation, which increases the possibility of a role for 4E-BP2 in repressing eIF4E phosphorylation. 4E-BP2 plays an important role in regulating translation initiation. Its down-regulation is associated with granulocytic maturation and the post-transcriptional regulation is associated with the early action of retinoic acid (RA).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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