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E6779 Millipore

EZview Red Anti-HA Affinity Gel

Synonym: Anti-HA

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Properties

Related Categories Alphabetical Index, Antibodies, H1-HE, HA, HA (Hemagglutinin),
application(s)   affinity chromatography: suitable
  immunoprecipitation (IP): suitable
storage temp.   2-8°C
Quality Level   200
shelf life   1 yr at 2‑8 °C
analyte chemical class(es)   proteins (hemagglutinin)
matrix   crosslinked agarose
capacity   ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

Description

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody covalently attached to crosslinked agarose beads.

Specificity

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Application

EZview Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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EZview™ Red Affinity Gels [VIDEO]

The EZview™ Red Affinity Gels have a vivid red dye that clearly defines the pellet from the supernatant. This enhanced visibility results in less tedious sample processing and greatly reduces the ris...
Keywords: Tagged proteins

Peer-Reviewed Papers
15

References

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