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  • E6781 - Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit

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E6781 Sigma-Aldrich

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-4E-T, Anti-Clast4, Anti-Eukaryotic translation initiation factor 4E nuclear import factor 1, Anti-Eukaryotic translation initiation factor 4E transporter, Anti-eIF4E-T

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies for RNA Regulation and Translation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μL using HeLa cell lysates
  indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde-fixed HEK-293T cells
  western blot: 1-2 μg/mL using HeLa cell lysates
species reactivity   human
mol wt   antigen 100 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q9NRA8
Gene Information   human ... EIF4ENIF1(56478)

Description

Immunogen

synthetic peptide corresponding amino acids 1-19 of human eIF4ENIF1, conjugated to KLH via a C-terminal added cysteine residue. This sequence is specific to human.

Application

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit is suitable for the following applications:
• Immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates
• Indirect immunofluorescence at a concentration of 5-10μg/mL using paraformaldehyde-fixed HEK-293T cells
• Western blotting at a concentration of 1-2μg/mL using HeLa cell lysates

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that bind the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. Its interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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E6656 Anti-eIF4ENIF1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

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