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  • E7025 - Anti-EphB1 antibody produced in goat

E7025 Sigma

Anti-EphB1 antibody produced in goat

affinity isolated antibody, lyophilized powder



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   rat
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   rat ... Ephb1(24338)
biological source   goat
conjugate   unconjugated



A recombinant rat EphB1 extracellular domain expressed in mouse NSO cells.

General description

Eph family of tyrosine kinase receptors mediates cell-cell attachment and aggregation by binding to specific ligands, ephrins. The Eph family consists of 14 structurally related receptors that are either membrane linked or are transmembrane with overlapping affinity for ligands. The transmembrane receptors EphA and EphB (ELK) subclasses signal via distinct mediators in the cytoplasm. EphB1 has several biological functions which includes neural synapsis formation and maturation, nervous system development and angiogenesis. It also interacts with the adaptor molecule Grb7 and regulates cell migration . Anti-EphB1 antibody can be used in western blotting. Goat anti-EphB1 antibody reacts specifically with recombinant rat EphB1. The antibody exhibits less than 5% (approx.) cross-reactivity with recombinant mouse EphB2, EphB3, EphB4, EphB6, EphA3, EphA4 and EphA7.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.


By immunoblotting and ELISA, the antibody shows <5% cross-reactivity with recombinant mouse EphB2, mouse EphB3, mouse EphB4, mouse EphB6, mouse EphA3, mouse EphA4, and mouse EphA7.


Anti-EphB1 antibody can be used in indirect ELISA (working concentration:- 0.5-1.0 μg/mL).

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