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E7158 Sigma-Aldrich

pFLAG-CMV-4 Expression Vector

shuttle vector for intracellular transient or stable expression of N-terminal Met-FLAG

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Properties

Related Categories Cloning and Expression, Core Bioreagents, Expression Vectors, For Stable Expression, Life Science Reagents for Transfection,
grade   for molecular biology
form   buffered aqueous solution
conjugate   Met-FLAG tagged
Peptide tag location   N-terminal
shipped in   dry ice
storage temp.   −20°C

Description

General description

pFLAG-CMV-4 is a 6.2 kb expression vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression of a properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein.

The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.

The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich.

pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

Vector Maps and Sequences

Components

• pFLAG-CMV-4 Expression Vector 20 μg (E1775) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pFLAG-CMV-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG®-fusion constructs. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

pFLAG-CMV4 is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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