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E7525 Sigma-Aldrich

Anti-Ephrin-B2 antibody produced in goat

affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   flow cytometry: 0.25 μg using 106 cells
  immunocytochemistry: 5-15 μg/mL
  western blot: 1 μg/mL
species reactivity   mouse
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P52800
Gene Information   mouse ... Efnb2(13642)

Description

General description

By immunoblotting and ELISA, the antibody shows ~50% cross-reactivity with recombinant human ephrin-B5 and 15% cross-reactivity with mouse ephrin-B1.

Immunogen

recombinant mouse ephrin-B2, expressed in NSO cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4 with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Ephrin B2 binds to EPH receptor B, a protein-tyrosine kinase family that belongs to the ephrin receptor subfamily. EphB receptors and EphrinB ligands have a regulatory effect on endothelial cell function, angiogenesis, migration, cell growth and survival.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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