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E7533 Sigma-Aldrich

p3XFLAG-CMV-7.1 Expression Vector

shuttle vector for transient expression of N-terminal 3xFLAG

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Properties

Related Categories Core Bioreagents, Expression Vectors, Life Science Reagents for Transfection, Research Essentials
grade   for molecular biology
form   buffered aqueous solution
quality   shuttle vector for transient expression of N-terminal 3xFLAG
conjugate   3X FLAG tagged
shipped in   dry ice
storage temp.   −20°C

Description

General description

The p3XFLAG-CMV-7.1 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish transient intracellular expression of N-terminal 3XFLAGfusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.


The p3XFLAG-CMV-7.1 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigenexpressing host.

The p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

For more information, please see Vector Maps and Sequences

Components

p3XFLAG-CMV-7.1 Expression Vector 20 μg (E4026) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
p3XFLAG-CMV-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs.

Legal Information

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.

3xFLAG is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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