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E8406 Sigma-Aldrich

Anti-EDEM1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-EDEM, Anti-ER degradation enhancer, mannosidase alpha-like 1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
  western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
species reactivity   human, mouse
mol wt   antigen 65 kDa (nonglycosylated human and mouse EDEM1)
  antigen 75 kDa (glycoslylated human and mouse EDEM1)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P20472
Gene Information   human ... EDEM1(9695)
mouse ... Pvalb(19293)

Description

General description

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.

Immunogen

synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1.

Application

Anti-EDEM1 antibody has been used in immunoblotting.

Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:
• Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
• Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
• Immunofluorescence Microscopy.
• Immunoblotting

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.

ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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E9906 Anti-EDEM2 (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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