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E8655 Sigma-Aldrich

Anti-E6AP antibody, Mouse monoclonal

clone E6AP-330, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to E1,E2,E3 Ligase,
conjugate   unconjugated
clone   E6AP-330, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using total cell extract from 293T cells
species reactivity   human, rat, mouse, monkey
mol wt   antigen ~100 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   O75461
Gene Information   human ... E2F6(1876)
mouse ... E2f6(50496)
rat ... E2f6(313978)

Description

General description

E6AP is an E3 ubiquitin ligase that is expressed by the UB3A gene. Inhibiton or alterations of the UB3A gene may cause a neurological disorder called the Angelman Syndrome. E6AP interacts with E1 and E2 enzymes to mediate ubiquitination of proteins marked for degradation. E6AP also binds with the E6 viral protein present in HPV-infected cells.
Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey.

Immunogen

human full-length recombinant E6AP.

Application

Monoclonal Anti-E6AP antibody is suitable for use in western blot (1-2 μg/mL using total cell extract from 293T cells), immunocytochemistry, immunoblotting, immunoprecipitation, indirect ELISA and microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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