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  • E8776 - Monoclonal Anti-E2F2 antibody produced in mouse

E8776 Sigma

Monoclonal Anti-E2F2 antibody produced in mouse

~1 mg/mL, clone CC11, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human
application(s)   immunoprecipitation (IP): suitable using native and denatured E2F2
  microarray: suitable
  western blot: 1-2 μg/mL using transfected 293T cells expressing E2F2
clone   CC11, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 53 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... E2F2(1870)
biological source   mouse
conjugate   unconjugated



recombinant human E2F2.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.


Monoclonal Anti-E2F2 antibody produced in mouse is suitable for the following applications:
• Immunoprecipitation using native and denatured E2F2
• Microarray
• Western blotting (at a concentration of 1-2μg/mL using transfected 293T cells expressing E2F2)
• May be used in various applications including electrophoretic mobility shift assay (EMSA)

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Biochem/physiol Actions

Transcription factor E2F2 is a protein encoded by the E2F2 gene in humans. It is aasociated with the regulation of cell-cycle and BP. E2F2 is a transcription factor that helps regulate the expression of a number of genes that are important in cell proliferation. The cell cycle progression is dependent on the transcriptional activity of E2F2 and is associated with pocket proteins that bind to DNA as a heterodimer of two distinct proteins. E2F-2 is functionally and immunologically associated with one of these two distinct proteins.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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