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  • E8781 - Anti-EDEM3 (C-terminal) antibody produced in rabbit

E8781 Sigma

Anti-EDEM3 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ER degradation enhancer, mannosidase alpha-like 3



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Oxidative Stress Proteins,
species reactivity   mouse, rat, human
application(s)   immunoprecipitation (IP): 1-2 μL using mouse 3T3 cell lysates
  western blot: 1:1,000-1:2,000 using whole extract of human HEK-293T cells
  western blot: 1:500-1:1,000 using whole extrct of rat PC12 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~120 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... EDEM3(80267)
mouse ... Edem3(66967)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 869-882 of human EDEM3, conjugated to KLH via an N-trerminal cysteine residue. The corresponding sequence is identical in mouse and rat EDEM3.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-EDEM3 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 1-2μL using mouse 3T3 cell lysates and western blotting (at a dilution of 1:1,000-1:2,000 using whole extract of human HEK-293T cells and at a dilution of 1:500-1:1,000 using whole extrct of rat PC12 cells).

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Biochem/physiol Actions

ER degradation-enhancing α-mannosidase-like 3 is an enzyme encoded by the EDEM3 gene in humans and consists of 931 amino acids. It consists of motifs of Class I α-mannosidases (glycosyl hydrolase family 47) in its N-terminal domain and a protease-associated motif in its C-terminal region. EDEM3 has α1,2-mannosidase activity. EDEM3 is also aasociated with folding-incompetent proteins carrying N-glycans that are extracted from futile folding cycles in the calnexin chaperone system.

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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