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E8901 Sigma-Aldrich

Monoclonal Anti-E2F1 antibody produced in mouse

~0.5 mg/mL, clone KH20, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors, Antibodies to Transcription Factors,
species reactivity   human, rat, Xenopus, mouse
application(s)   immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: 1-2 μg/mL using transfected 293T cells expressing E2F1
clone   KH20, monoclonal
concentration   ~0.5 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt 53 kDa (may vary in a recombinant preparation)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... E2F1(1869)
mouse ... E2f1(13555)
rat ... E2f1(399489)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   Q01094

Description

Immunogen

recombinant human E2F1.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity

The epitope recognized by the antibody resides in the N-terminal region of human E2F1 (amino acids 1-89). May be used for immunoprecipitation (native and denatured), immunoblotting immunocytochemistry, and gel mobility shift assay.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Monoclonal Anti-E2F1 antibody produced in mouse is suitable for the following applications:
• Immunoprecipitation
• Microarray
• Western blotting (1-2μg/mL using transfected 293T cells expressing E2F1)

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Transcription factor E2F1 is a protein encoded by the E2F1 gene in humans and belongs to E2F family of transcription factors. It is located on chromosome 20q11 and plays a critical role in the transcription of certain genes required for cell cycle progression. E2F1 may facilitate identification of additional E2F family members and detection of E2F1 abnormalities in human tumors. It plays a crutial role in regulating cell cycle and other biological processes in cells. E2F-1 gene expression is higher in rectal cancer tissue compared to normal tissue.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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