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ECM001 Sigma-Aldrich

Fibronectin human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonym: CIG, Cold-insoluble globulin, FN, Fibronectin

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Properties

Quality Level   200
biological source   human
recombinant   expressed in HEK 293 cells
sterility   sterile
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
mol wt   259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)
packaging   pkg of 200 μg
application(s)   cell culture | mammalian: suitable
impurities   ≤1 EU/μg endotoxin, tested
shipped in   ambient
storage temp.   −20°C
Gene Information   human ... FN1(2335)

Description

General description

Recombinant human fibronectin is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 259.5 kDa. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Biochem/physiol Actions

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36.1 FN exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. This product is a recombinant form of the plasma FN (uniprot number P02751). It contains no tags and is identical in sequence to the native plasma FN. Plasma FN is a major protein component of blood plasma (300 μg/ml) and is produced in the liver by hepatocytes.

Physical form

Supplied as a powder, lyophilized from CAPS buffered saline.

Analysis Note

The biological activity of recombinant human fibronectin was tested in culture by measuring the ability of immobilized fibronectin to support adhesion of CHO cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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