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EPI004 Sigma-Aldrich

Histone Deacetylase 3 (HDAC3) Activity Assay Kit

100 assays in 96 well plates



Related Categories Acetylation and Decacetylation Assays, Epigenetics, Kits for Epigenetics, Molecular Biology
usage   100 assays in 96 well plates
NCBI accession no.   NM_003883 (Human)
  NP_034541 (Mouse)
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... HDAC3(8841)
mouse ... HDAC3(15183)


General description

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, it plays a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.

With Sigma′s HDAC3 Activity Assay Kit, HDAC3 present in a test sample will act with the supplied Developer, to deacetylate and then cleave the HDAC3 Substrate [R-H-K-K(Ac)-AFC]. This activity will release the quenched fluorescent group, AFC, which can be detected at Em/Ex = 380/500 nm. Trichostatin A is an HDAC inhibitor included in the kit to verify HDAC3 activity. The kit provides a rapid, simple, sensitive and reliable test. It is suitable for either individual tests or high throughput assays, from nuclear extracts, purified, or immunoprecipitated HDAC3, and from native, recombinant, or genetically modified HDAC3.

Features and Benefits

• Simple, sensitive, and reliable assay
• Simple procedure; takes ~60 min
• Utilizes fluorometric methods
• Sample type: cell and tissue lysates, plasma and serum, other biological fluids
• Species reactivity: mammalian
• Suitable for individual tests or high throughput assays and kinetic studies
• Convenient 96-well microplate format
• Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples

Safety & Documentation

Safety Information

UN 1993 / PGIII
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup


Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers


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