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EPI005 Sigma-Aldrich

Histone Deacetylase 3 (HDAC3) Inhibitor Screening Kit

100 assays in 96 well plates



Related Categories Acetylation and Decacetylation Assays, Epigenetics, Kits for Epigenetics, Molecular Biology
Quality Level   100
usage   100 assays in 96 well plates
NCBI accession no.   NM_003883 (Human)
  NP_034541 (Mouse)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HDAC3(8841)
mouse ... HDAC3(15183)


General description

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. Of the several classes of HDACs, this kit assays a member of the zinc-independent, NADH requiring class, HDAC3. With Sigma′s HDAC3 Inhibitor Screening Kit, HDAC3 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC3 Substrate [Arg-His-Lys-Lys(Ac)-AFC]. This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC3 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.

Features and Benefits

• Simple, sensitive, and reliable assay
• Simple procedure; takes ~60 min
• Utilizes fluorometric methods
• Sample type: candidate HDAC3 inhibitors
• Suitable for screening HDAC3 inhibitors
• Suitable for individual tests or high throughput assays and kinetic studies
• Convenient 96-well microplate format

Other Notes

The kit is shipped on dry ice. Store HDAC-3 enzyme at -80 °C upon receipt. All other components should be stored at -20 °C, protected from light. All -20 °C reagents should be used within 2 months after thawing.

Safety & Documentation

Safety Information

UN 1993 / PGIII
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup


Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers


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