• USA Home
  • EPI005 - Histone Deacetylase 3 (HDAC3) Inhibitor Screening Kit

EMAIL THIS PAGE TO A FRIEND
EPI005 Sigma-Aldrich

Histone Deacetylase 3 (HDAC3) Inhibitor Screening Kit

100 assays in 96 well plates

Purchase

Properties

Related Categories Acetylation and Decacetylation Assays, Epigenetics, Kits for Epigenetics, Molecular Biology
usage   100 assays in 96 well plates
NCBI accession no.   NM_003883 (Human)
  NP_034541 (Mouse)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HDAC3(8841)
mouse ... HDAC3(15183)

Description

General description

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. Of the several classes of HDACs, this kit assays a member of the zinc-independent, NADH requiring class, HDAC3. With Sigma′s HDAC3 Inhibitor Screening Kit, HDAC3 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC3 Substrate [Arg-His-Lys-Lys(Ac)-AFC]. This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC3 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.

Features and Benefits

• Simple, sensitive, and reliable assay
• Simple procedure; takes ~60 min
• Utilizes fluorometric methods
• Sample type: candidate HDAC3 inhibitors
• Suitable for screening HDAC3 inhibitors
• Suitable for individual tests or high throughput assays and kinetic studies
• Convenient 96-well microplate format

Other Notes

The kit is shipped on dry ice. Store HDAC-3 enzyme at -80 °C upon receipt. All other components should be stored at -20 °C, protected from light. All -20 °C reagents should be used within 2 months after thawing.

Safety & Documentation

Safety Information

RIDADR 
UN 1993 / PGIII
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

390585 Suberohydroxamic acid, 95%
S4068 Splitomicin, ≥98% (HPLC), powder
S7817 Scriptaid, ≥95%, solid
A2478 APHA Compound 8, ≥98% (HPLC), solid
O3139 Oxamflatin, ≥98% (HPLC), solid
B4061 BATCP, ≥98% (HPLC), solid
P5874 PTACH, ≥98% (HPLC), solid
M2195 MOCPAC, ≥95% (HPLC), solid
M5820 M344, ≥98% (HPLC), powder
T1952 Trichostatin A, Ready Made Solution, 5 mM in DMSO (0.2 μm-filtered), from Streptomyces sp.
C0494 Cambinol, ≥97% (HPLC), white powder
C0621 CI−994, ≥98% (HPLC), powder
T2580 Trapoxin A, ≥98% (HPLC), from Helicoma ambiens
T8552 Trichostatin A, ≥98% (HPLC), from Streptomyces sp.
P4543 Valproic acid sodium salt, 98%
EPS002 MS-275, A HDAC1 and HDAC3 inhibitor
EPS003 CUDC 101, A potent inhibitor of HDACs and receptor tyrosine kinases
B5887 Sodium butyrate, ≥98.5% (GC)
R5010 Resveratrol, ≥99% (HPLC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?