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EPI010 Sigma-Aldrich

Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit

100 assays in 96 well plates

  •  NACRES NA.41

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Properties

Related Categories Acetylation and Decacetylation Assays, Epigenetics, Kits for Epigenetics, Molecular Biology
usage   100 assays in 96 well plates
storage condition   protect from light
NCBI accession no.   NM_001122765 (Mouse)
  NM_001193286 (Human)
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... SIRT2(22933)
mouse ... SIRT2(64383)

Description

General description

Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins. Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules. The gene encoding this protein is localized on human chromosome 19q13.2.

With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.

Application

Sirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay.

Biochem/physiol Actions

Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. Huntington′s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.

Features and Benefits

• Simple, sensitive, and reliable assay
• Species reactivity: mammalian
• Utilizes fluorometric methods
• Suitable for individual tests or high throughput assays
• Convenient 96-well microplate format
• Screening and characterization of Sirtuin inhibitors

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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