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ESPCAS9PRO Sigma-Aldrich

eSpCas9 Protein

from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS

Synonym: Enhanced specificity Cas9, eCas9, eSpCas9 1.1, eSpCas9, eSpyCas9

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Properties

Related Categories CRISPR-Cas9, Functional Genomics and RNAi, MISSION™ Cas9 Proteins, Molecular Biology
Quality Level   PREMIUM
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
application(s)   CRISPR: suitable
shipped in   ambient
storage temp.   −20°C

Description

Components

Each kit consists of:
• one vial of eSpCas9 recombinant protein
• one vial containing 1 mL of 1× dilution buffer
• one vial containing 1 mL of nuclease-free water with glycerol

Features and Benefits

• Enhanced specificity compared to wild type Cas9
• Highly active
• Ready-to-inject/transfect

Other Notes

Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

Packaging

pkg of 50 μg
pkg of 250 μg

Legal Information

CRISPR Use License Agreement

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Reconstitution

Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. The solution should be allowed to incubate at room temperature (20-23 °C) for 10 minutes. Centrifuge immediately before use.

Application

Functional Genomics/Target Validation/Genome Editing

Principle

Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.

General description

Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

Kit component only

Description

Product #

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Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli    
Dilution buffer for Cas9 proteins    
Reconstitution solution for Cas9 proteins    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Spring into Genome Editing
Protocols & Articles

Protocols

Application Note: Synthetic CRISPR - Cas9 Ribonucleoprotein (RNP)

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA. In th...
Keywords: Cell culture, Centrifugation, Culture media, Degradations, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Transfection

Related Content

CRISPR

In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally fo...
Keywords: Genetic, Transduction, Transfection

Peer-Reviewed Papers
15

References

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