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ESPCAS9PRO Sigma

ESPCAS9 Protein

Synonym: Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli

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Properties

Related Categories CRISPR-Cas9, Cas9 Protein, Functional Genomics and RNAi, Molecular Biology
Quality Level   PREMIUM
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
shipped in   ambient
storage temp.   −20°C

Description

Components

Each kit consists of:
• one vial of eSpCas9 recombinant protein
• one vial containing 1 mL of 1× dilution buffer
• one vial containing 1 mL of nuclease-free water with glycerol

Features and Benefits

• Enhanced specificity compared to wild type Cas9
• Highly active
• Ready-to-inject/transfect

Other Notes

Use our CRISPR Selection Tool to order gRNA

Packaging

pkg of 50 μg
pkg of 250 μg

Legal Information

CRISPR Use License Agreement

Reconstitution

Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. The solution should be allowed to incubate at room temperature (20-23 °C) for 10 minutes. Centrifuge immediately before use.

Application

Functional Genomics/Target Validation/Genome Editing

Principle

Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.

General description

Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

Kit component only

Description

Product #

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Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli SDS    
Dilution buffer for Cas9 proteins SDS    
Reconstitution solution for Cas9 proteins SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Protocols

Protocol Guide: CRISPR/CAS9 Gene Editing of Human Induced Pluripotent Stem Cells (iPSCs)

Induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny arising from of all germ layers of the body including: ectoderm, endoderm, and mesoderm. The ability t...
Keywords: Alzheimer Disease, Apoptosis, Cell culture, Centrifugation, Cloning, Culture media, Gene expression, Genetic, Microscopy, Parkinson Disease, Phase transitions, Transfection

Peer-Reviewed Papers
15

References

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