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  • F0300 - Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

F0300 Sigma

Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-FGFR-2



Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections
  western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 115-125 kDa (doublet)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FGFR2(2263)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 809-821 of the cytoplasmic region of human FGFR-2 (bek).

General description

No reaction with FGFR-1(flg) and FGFR-3 is detected.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit was used for immunohistochemistry of free-floating rat brain tissue sections.

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Biochem/physiol Actions

Fibroblast growth factors (FGFs) and their receptors, FGFRs, are important regulators of cell proliferation, migration, tissue repair and differentiation during vertebrate development. FGFR-2 isoforms are expressed exclusively on cells of epithelial origin and are involved in tumor progression in thyroid cancers. Abnormalities in FGFR-2 expressions have been observed in skin, bone and cancer pathologies.

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Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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