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F0635 Sigma-Aldrich

Fibronectin rat plasma

powder, BioReagent, suitable for cell culture

Synonym: Fibronectin

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Description

General description

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

Application

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.

Biochem/physiol Actions

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

Components

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Analysis Note

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

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Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurall...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

What is the Extracellular Matrix?

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Peer-Reviewed Papers
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References

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