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F0767 Sigma-Aldrich

Monoclonal Anti-Human IgG1−FITC antibody produced in mouse

clone 8c/6-39, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Human IgG1 (Fc specific)

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, FITC Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,
conjugate   FITC conjugate
clone   8c/6-39, monoclonal
biological source   mouse
application(s)   dot immunobinding: 1:64
  particle immunofluorescence: 1:32
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   secondary antibodies
storage condition   protect from light

Description

General description

Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies.

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG1−FITC antibody produced in mouse has been used in flow cytometry. It has also been used in direct hemagglutination (HA) and hemagglutination inhibition

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

IgG1 and IgG3 have an ability to adhere to the mononuclear phagocytes. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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