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F0895 Sigma-Aldrich

Fibronectin human plasma

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

Synonym: Fibronectin

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Description

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Application

Fibronectin human plasma has been used in the determination of H2O2 consumption rate. It has also been used in coating the Sylgard® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis.

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should I store and handle Product F0895, Fibronectin from human plasma?
Recommended storage of this material is2-8 °C.Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
Is Product F0895, Fibronectin from human plasma, sterile?
Fibronectin 0.1% solution is a sterile product.It is tested for sterility.The sterility test results can be found on the lot-specific certificate of analysis.
What is the solvent for Product F0895, Fibronectin from human plasma?
This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
How much Product F0895, Fibronectin from human plasma, do I use for my application?
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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