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F1141 Sigma-Aldrich

Fibronectin bovine plasma

solution, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Fibronectin

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Description

General description

Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.

Application

Fibronectin (FN) bovine plasma has been used in:
• fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
• transwell migration
• fibronectin assay

Biochem/physiol Actions

Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Other Notes

Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
The species source for the fibronectin used when coating culture surfaces is not important.It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
How long can I store the fibronectin solution?
The product is good for at least a year at 2-8 °C if kept sterile.
How long can I store fibronectin coated plates, etc?
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
What is the procedure for coating culture surfaces with fibronectin?
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.2. Allow to air dry for at least 45 minutes at room temperature.Excess fibronectin may be removed by aspiration, but this is not necessary.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Cell Culture Guide
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Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
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