• USA Home
  • F135 - Fractalkine, Extracellular Domain human

EMAIL THIS PAGE TO A FRIEND
F135 Sigma-Aldrich

Fractalkine, Extracellular Domain human

≥97% (SDS-PAGE), recombinant, expressed in NSO cells, suitable for cell culture, lyophilized powder

Synonym: CX3CL-1, Neurotactin

Purchase

Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Chemokines, Cytokines and Growth Factors,
Quality Level   200
biological source   human
recombinant   expressed in NSO cells
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
potency   22.62 ng/mL
mol wt   ~90 kDa
packaging   pkg of 25 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
UniProt accession no.   P78423
storage temp.   −20°C
Gene Information   human ... CX3CL1(6376)

Description

General description

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine is a membrane protein, which is the only member of CXC3 family. Its chemokine domain (CKD) anchors it to the cell membrane through its mucin-like stalk. It acts as a ligand for the G-protein coupled receptor CX3CR1, which is found on smooth muscle cells, T-cells, natural killer (NK) cells and monocytes. Fractalkine is expressed on epithelial, endothelial and smooth muscle cells and neurons.

Biochem/physiol Actions

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine, along with CX3CR1 is involved in various disorders such as atherosclerosis, multiple sclerosis, neuropathic pain and rheumatic disorders. It facilitates the proliferation and survival of primary human vascular smooth muscle cells in an epidermal growth factor receptor (EGR)-dependent manner. It plays an essential role in cell survival and promotes the survival of cells such as monocytes, T-cells and microglia. In humans, it is thought to be involved in atherogenesis, and thus, might have potential as a target in treatment of cardiovascular disease. In patients with sickle cell disease (SCD), the serum levels of this chemokine is increased, thus implicating it in the pathogenesis of inflammation associated with SCD.

Physical form

Lyophilized from a 0.2 μm-filtered solution in PBS containing 50 μg bovine serum albumin per 1 μg of cytokine

Analysis Note

Biological activity is measured by its ability to chemoattract freshly isolated peripheral blood lymphocytes or mouse BaF/3 cells transfected with hCX3CR-1.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Request a Serum Sample

Pipette Controller from Brand
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?