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F1392 Sigma-Aldrich

Riboflavin 5′-monophosphate sodium salt hydrate

for electrophoresis, suitable for acrylamide photopolymerization, ≥70%, powder

Synonym: FMN-Na, Flavin mononucleotide, Riboflavin 5′-phosphate sodium salt

  • CAS Number 130-40-5 (anhydrous)

  • Empirical Formula (Hill Notation) C17H20N4NaO9P · xH2O

  • Molecular Weight 478.33 (anhydrous basis)

  •  Beilstein/REAXYS Number 4106529

  •  EC Number 204-988-6

  •  MDL number MFCD00150992

  •  PubChem Substance ID 24894764

  •  NACRES NA.51



Related Categories Biochemicals and Reagents, Nucleosides, Nucleotides, Oligonucleotides, Nucleotides More...
grade   for electrophoresis
assay   ≥70%
form   powder
optical activity   [α]20/D +37 to +42°, c = 1.5 in 5 M HCl(lit.)
solubility   H2O: soluble 50 mg/mL, clear, orange
ε (extinction coefficient)   12.5 at 445 nm at 1 mM
suitability   suitable for acrylamide photopolymerization
foreign activity   Protease, none detected
storage temp.   −20°C
SMILES string   O.[Na+].Cc1cc2N=C3C(=O)NC(=O)N=C3N(C[C@H](O)[C@H](O)[C@H](O)COP(O)([O-])=O)c2cc1C
InChI   1S/C17H21N4O9P.Na.H2O/c1-7-3-9-10(4-8(7)2)21(15-13(18-9)16(25)20-17(26)19-15)5-11(22)14(24)12(23)6-30-31(27,28)29;;/h3-4,11-12,14,22-24H,5-6H2,1-2H3,(H,20,25,26)(H2,27,28,29);;1H2/q;+1;/p-1/t11-,12+,14-;;/m0../s1



FMN is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. FMN photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen, but traces of oxygen allow for leucoflavin to reoxidize with free-radical generation. The catalysts, TEMED or DMAPN, are commonly added to speed up the free radical formation. Free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. FMN is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of FMN over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.
FMN from Sigma was used:
• to study its mechanism of inhibition of mutagenicity of a benzo[a]pyrene 7,8-diol 9,10-epoxide, a carcinogenic metabolite of benzo[a]pyrene
• along with flavin adenine dinucleotide for reactivation of the enzyme that catalyzes the oxidation of pyridoxamine to pyridoxal in a study
• to study the reduction of polymeric azo and nitro dyes by intestinal bacteria
• to study the effect of FMN on ferrisiderophore reductase activity in the cytoplasmic fraction of Agrobacterium tumefaciens.


25 mg in poly bottle

Biochem/physiol Actions

FMN is a coenzyme synthesized from riboflavin (vitamin B2). It acts as a component of complex I of the electron transport chain. The enzymes containing FMN are called as flavin enzymes and are involved in oxidation-reduction mechanisms.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


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