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F1429 Sigma-Aldrich

Anti-Fe65 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, FB-FJ, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable using mouse brain lysate
species reactivity   mouse
mol wt   antigen 97 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q9QXJ1
Gene Information   mouse ... Apbb1(11785)

Description

Specificity

The antibody does not detect endogenous levels of Fe65.

Immunogen

synthetic peptide corresponding to amino acid residues 352-369 from mouse Fe65.

Application

Anti-Fe65 antibody produced in rabbit is suitable for western blotting using mouse brain lysate.

Physical form

Solution in phosphate buffered saline containing 1.0 mg/mL bovine serum albumin and 0.05% sodium azide.

Biochem/physiol Actions

Amyloid β A4 precursor protein-binding family B member 1 is a protein encoded by the APBB1 gene in humans and is mapped on human metaphase chromosomes to band 11p15. Fe65 is a neuronal adaptor protein that is involved in brain development, Alzheimer disease amyloid precursor protein (APP) signaling and proteolytic processing of APP. It participates in various processes by recruiting various interacting partners to form functional complexes and acts as a novel regulator of neurite outgrowth. Fe65 significantly alters actin dynamics and mitochondrial function in neuronal cells.

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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