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  • F1527 - Monoclonal Anti-CD45RA−FITC antibody produced in mouse

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F1527 Sigma-Aldrich

Monoclonal Anti-CD45RA−FITC antibody produced in mouse

clone F8-11-13, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   FITC conjugate
clone   F8-11-13, monoclonal
biological source   mouse
application(s)   flow cytometry: 10 μL using 1 × 106 cells
species reactivity   human
mol wt   antigen 220 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P08575
Gene Information   human ... PTPRC(5788)

Description

Immunogen

fresh human lymph node lymphocytes depleted of surface Ig-positive cells

Target description

This antigen is a single chain glycoprotein, which is expressed on B cells, monocytes and T cell subsets.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Safety & Documentation

Safety Information

WGK Germany 
-

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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