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F1804 Millipore

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym: Anti-ddddk, Anti-dykddddk, flag antibody

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Properties

Related Categories Alphabetical Index, Antibodies, FK-FO, FLAG Antibodies, FLAG System,
biological source   mouse
antibody form   affinity isolated antibody
antibody product type   primary antibodies
clone   M2, monoclonal
form   buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by   affinity chromatography (affinity tag purification)
species reactivity   all
concentration   1 mg/mL
isotype   IgG1
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   wet ice
storage temp.   −20°C

Description

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG® Literature portal.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Same Products - New Packaging
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research. After electrophoresis, proteins are immobilized on a nitrocellulose or PVDF membrane and then probed with a...
BioFiles 2011, 6.3, 16.
Keywords: Catalytic combustion detector, Electrophoresis, Immobilization, PAGE, Proteomics, Western blot

Protocols

Cell-type specific sequencing of microRNAs from complex animal tissues

Chiara Alberti a , Raphael A Manzenreither b , Ivica Sowemimo b , Thomas R Burkard a, b , Jingkui Wang a , Katharina Mahofsky a , Stefan L Ameres b , Luisa Cochella a
Keywords: AGE, Affinity chromatography, Amplification, Catalysis, Cell disruption, Cell signaling, Centrifugation, Chromatography, Cloning, Electrophoresis, Eliminations, Enzyme activity, Gel electrophoresis, Gene expression, Genetics, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Metabolism, Methylations, Microscopy, Northern blot, Nucleic acid hybridization, Oxidations, PAGE, Peptide synthesis, Polymerase chain reaction, Precipitation, Protein assay, Purification, Recombination, Sequencing, Titrations, Transcription, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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