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  • F1804 - Monoclonal ANTI-FLAG® M2 antibody produced in mouse

F1804 Millipore

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym: Anti-ddddk, Anti-dykddddk, flag antibody



Related Categories Alphabetical Index, Antibodies, FK-FO, FLAG Antibodies, FLAG System,
biological source   mouse
antibody form   affinity isolated antibody
antibody product type   primary antibodies
clone   M2, monoclonal
form   buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by   affinity chromatography (affinity tag purification)
species reactivity   all
concentration   1 mg/mL
isotype   IgG1
immunogen sequence   DYKDDDDK
conjugate   unconjugated
shipped in   wet ice
storage temp.   −20°C


General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification


FLAG; peptide sequence DYKDDDDK

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk


Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.


For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG® Literature portal.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Same Products - New Packaging
Protocols & Articles


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Keywords: AGE, Affinity chromatography, Amplification, Catalysis, Cell disruption, Cell signaling, Centrifugation, Chromatography, Cloning, Electrophoresis, Eliminations, Enzyme activity, Gel electrophoresis, Gene expression, Genetics, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Metabolism, Methylations, Microscopy, Northern blot, Nucleic acid hybridization, Oxidations, PAGE, Peptide synthesis, Polymerase chain reaction, Precipitation, Protein assay, Purification, Recombination, Sequencing, Titrations, Transcription, Western blot

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