• USA Home
  • F2051 - Monoclonal Anti-Fas Ligand antibody produced in mouse

F2051 Sigma

Monoclonal Anti-Fas Ligand antibody produced in mouse

~1 mg/mL, clone Alf2.1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-CD95L, Anti-FasL



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   flow cytometry: 1-2 μg/test using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, stably-transfected with human Fas Ligand)
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
clone   Alf2.1, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 24-40 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FASLG(356)
biological source   mouse
conjugate   unconjugated



recombinant soluble active extracellular domain of human Fas ligand.

General description

The Fas ligand (Fas ligand, FasL, CD95L) is an integral membrane protein and is a type II transmembrane glycoprotein expressed by CD4+ and CD8+ cytotoxic T lymphocytes, natural killer and a small number of non-lymphoid cells. FasL is a member of the TNF family. Membrane bound FasL (mFasL) is a 40kDa protein, while the active soluble form of FasL (sFasL) was identified as a 26kDa protein. FasL has four potential N-glycosylation sites.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor


Monoclonal Anti-Fas Ligand antibody is suitable for flow cytometry at a concentration of 1-2μg/test using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, stably-transfected with human Fas Ligand). It is also suitable for immunocytochemistry; immunoprecipitation; indirect ELISA, microarray analysis.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Biochem/physiol Actions

Fas ligand (FasL) binds to the Fas receptor and triggers apoptosis, which plays a crucial role in the maintenance of immune system homeostasis. Fas/FasL play crucial role in carcinogenesis, tumor outgrowth, and metastasis. Mutation in FasL is associated with systemic lupus erythematosus.

Price and Availability

Antibody Explorer

Explore with Confidence

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

WGK Germany  -
Protocols & Articles


Antibodies to Fas (CD95/Apo-1) and Fas Ligand (CD95L)

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing prot...
Keywords: Apoptosis, Ligands

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?