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F2055 Sigma

Monoclonal Anti-hFBXW7 antibody produced in mouse

clone FB407, purified immunoglobulin

Synonym: Anti-ARCHIPELAGO (Drosophila), Anti-Cdc4 (S. serevisia), Anti-F-box and WD40 domain protein 7, Anti-FBW7, Anti-SEL-10 (C. elegance)

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
species reactivity   human, rat, mouse
application(s)   immunocytochemistry: 10-20 μg/mL using human A431 or HeLa cells
  indirect ELISA: suitable
  western blot: 5-10 μg/mL using human A431 cell extracts
clone   FB407, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2a
mol wt   antigen mol wt ~60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... FBXW7(55294)
mouse ... Fbxw7(50754)
biological source   mouse
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino-acids 238-250 of human hFBXW7 isoforms α, β, and γ.

General description

FBXW7 is a member of F-box protein family, which acts as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). The proteins have a bipartite structure. The shared F-box motif links F-box protein to Skp1 and the core complex, whereas divergent protein-protein interaction motifs selectively bind their cognate substrates. There are three FBXW7 isoforms α, β and γ that share 10 out of 11 exons but they differ in their subcellular localization as well as in substrate recognition activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes human, mouse, and rat hFBXW7.

Application

Monoclonal hFBXW7 antibody is suitable for immunoblotting, at a concentration of 5-10mg/mL using human A431 cells, indirect ELISA and immunocytochemistry at a concentration of 10-20mg/mL using human A431 cells.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

F-box proteins is associated with various signalling pathways, such as nutrient sensing in yeast,conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch, leading to their ubiquitination and degradation. Furthermore, it was found mutated in breast, endometrial, ovarian, and colon cancer, and finally results in genomic instability. Similar to the inactivation mode of other known tumor suppressors, the SV40 large T antigen binds FBXW7 without detectible effects on its stability, and thus acts as an inhibitor of FBXW7 activity.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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