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F2302 Sigma-Aldrich

Fractalkine, Chemokine Domain from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym: CX3CL-1, Neurotactin

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Chemokines, Cytokines and Growth Factors,
Quality Level   100
biological source   mouse
recombinant   expressed in E. coli
assay   >97% (SDS-PAGE)
form   lyophilized powder
potency   0.3-3 μg/mL
mol wt   calculated mol wt ~9.5 kDa
packaging   pkg of 25 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
UniProt accession no.   O35188
storage temp.   −20°C
Gene Information   mouse ... Cx3cl1(20312)

Description

General description

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine is a membrane protein, which is the only member of CXC3 family. Its chemokine domain (CKD) anchors it to the cell membrane through its mucin-like stalk. It acts as a ligand for the G-protein coupled receptor CX3CR1, which is found on smooth muscle cells, T-cells, natural killer (NK) cells and monocytes. Fractalkine is expressed on epithelial, endothelial and smooth muscle cells and neurons.

Biochem/physiol Actions

CX3CL1 (chemokine (C-X3-C motif) ligand 1) or fractalkine, along with CX3CR1 is involved in various disorders such as atherosclerosis, multiple sclerosis, neuropathic pain and rheumatic disorders. It facilitates the proliferation and survival of primary human vascular smooth muscle cells in an epidermal growth factor receptor (EGR)-dependent manner. It plays an essential role in cell survival and promotes the survival of cells such as monocytes, T-cells and microglia. In humans, it is thought to be involved in atherogenesis, and thus, might have potential as a target in treatment of cardiovascular disease. In patients with sickle cell disease (SCD), the serum levels of this chemokine is increased, thus implicating it in the pathogenesis of inflammation associated with SCD.

Leukocyte chemoattractant protein and member of the chemokine superfamily possessing a CX3C motif. Mouse fractalkine is chemotactic for neutrophils and T-lymphocytes but not monocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to chemoattract freshly isolated peripheral blood lymphocytes.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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