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F2518 Sigma-Aldrich

Fibronectin human foreskin fibroblasts

lyophilized powder, BioReagent, suitable for cell culture

Synonym: Fibronectin

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Description

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.

Application

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Packaging

0.5 mg in serum bottle

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Caution

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

dialyzed against chloroform

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

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Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
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Fibronectin (FN)

Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurall...
George Sitterley
BioFiles 2008, 3.8, 8.
Keywords: Adhesion, Cell culture, transformation

Fibronectin Cell Attachment Protocol

Freezing and thawing of reconstituted fibronectin is not recommended as breakdown of protein will occur.
George Sitterley
BioFiles 2008, 3.8, 9.
Keywords: Phase transitions

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Peer-Reviewed Papers
15

References

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