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  • F2645 - Anti-Factor IX antibody, Mouse monoclonal

F2645 Sigma

Anti-Factor IX antibody, Mouse monoclonal

clone HIX-1, purified from hybridma cell culture

Synonym: Monoclonal Anti-Factor IX



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood, Antibodies to Clotting Factors,
species reactivity   human
application(s)   indirect ELISA: suitable
  western blot: 2-4 μg/mL
clone   HIX-1, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... F9(2158)
biological source   mouse
conjugate   unconjugated



Factor IX from pooled normal human plasma.

General description

Factor IX (FIX) is a 55 kDa, single chain vitamin K-dependent plasma zymogen which plays a key role in the intrinsic and extrinsic blood coagulation systems. In mature stage of FIX, it comprises of four distinct domains: the N-terminal Gla-domain, the EGF-like domain, the activating peptide (AP) domain and the C-terminal serine protease domain. Hereditary deficiencies or dysfunctions of factor IX cause hemophilia B or "Christmas Disease" (the surname of the first family described). In haemophilia B, FIX showed impaired coagulation and increased tendency to bleed as a reason of mutation in the X chromosome linked FIX gene. Factor IX is synthesized in liver parenchymal cells and requires a post-translational vitamin K-dependent modification in order to become a mature plasma zymogen. When patients lack vitamin-K or take oral anticoagulants that interfere with the metabolism of vitamin-K, a hypocoagulable or antithrombotic state is induced.

Physical form

Solution in 10 mM HEPES, pH 7.4, with 140 mM sodium chloride and 0.05% sodium azide.


Monoclonal Anti-Factor IX, a divalent cation-independent antibody, recognizes factor IX when used on immunoblots of non-denatured, non-reduced human plasma. It is also useful as paired labeled antibody in sandwich-type immunoassays with Monoclonal anti-Factor IX, clone HIX-5 (Product No. F1020).

This antibody may be used for purification of Factor IX and preparation of Factor IX depleted human plasma.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Monoclonal Anti-Factor IX antibody produced in mouse is suitable for ELISA-based assay in detection of FIX Inhibitors and it has been used as a positive control in ELISA. It is also suitable for western blot at a concentration of 2-4μg/mL.

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Biochem/physiol Actions

FIX plays a key role in the intrinsic and extrinsic blood coagulation systems. It is synthesized in liver and later upon activation in plasma it gets converted into a serine protease. In second step, FIX undergoes series of complex post-translation modifications such as γ-carboxylation of 12 N-terminal glutamic acid residues, N- and O-linked glycosylation, phosphorylation, β-hydroxylation, sulfation, disulfide bond formation etc. It is secreted into the plasma after completion of post-translation modifications.

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