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F2648 Sigma-Aldrich

Monoclonal Anti-CD11b−FITC antibody produced in mouse

clone ICRF44, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD11b

  •  NACRES NA.44

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   FITC conjugate
clone   ICRF44, monoclonal
biological source   mouse
application(s)   flow cytometry: 10 μL using 1 × 106 cells
species reactivity   human
mol wt   antigen 165-170 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
storage condition   protect from light
UniProt accession no.   P11215
Gene Information   human ... ITGAM(3684)

Description

General description

CD11b is an α/β heterodimeric glycoprotein. It belongs to the β2 integrin family and also referred as Mac-1, CR3, MO-1, and C3bi receptor.

Monoclonal Anti-Human CD11b antibody (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with the human monocytes. CD11b is expressed on the surface of circulating monocytes, granulocytes, and certain natural killer (NK) cells. It is also present on subsets of macrophages. CD11b is present in subcellular granules and is translocated to the surface after activation.

Specificity

Recognizes the CD11b α-chain of the human CD11b/CD18 complex. The antibody is useful for studies on cell adhesion and migration and for the detection of leukocyte adhesion deficiencies. It may be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.
2nd Workshop: code no. M104
3rd Workshop: code no. 202 and 238
4th Workshop: code no. M47

Immunogen

human monocytes.

Application

Anti-CD11b-FITC antibody is suitable for flow cytometric analysis of CD11b expression in RA-induced differentiation of human promyelocytic leukemia cells. It is also suitable for flow cytometric analysis of spleen cells to study the beneficial effects of dietary consumption of n-3 polyunsaturated fatty acids (PUFA) and two selective estrogen receptor modulator (SERM) derivatives.

Target description

CD11b α-chain of the human CD11b/CD18 complex is an α/β heterodimeric glycoprotein which belongs to the β2 integrin family. It is also known as Mac-1, CR3, MO-1 and C3bi receptor. CD11b is expressed on the surface of circulating monocytes, granulocytes and certain NK cells and is also present in subsets of macrophages. It functions as a receptor for iC3b, clotting factor X, fibrinogen and ICAM-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Anti-CD11b-FITC antibody is expressed on the surface of circulating monocytes, granulocytes, and certain NK cells. It is also present on subsets of macrophages and in subcellular granules of granulocytes. Upon activation, it translocates to the surface. It forms a CD11b/CD18 complex to participate in a variety of cell-cell and cell-substrate interactions such as attachment and phagocytosis of particles coated with C3bi by granulocytes and macrophages and phagocytosis of opsonized pathogens. It also plays a role in the initiation of a coagulation protease cascade and in cell migration mechanisms.

Surface expression of cluster of differentiation molecule CD11b/CD18 is capable of both functional and quantitative upregulation. CD11b/CD18 functions as a receptor for C3bi, clotting factor X, fibrinogen, and intercellular adhesion molecule 1 (ICAM-1). CD11b/CD18 is involved in a variety of cell-cell and cell-substrate interactions such as attachment and phagocytosis of particles coated with complement receptor type 3 (C3bi) by granulocytes and macrophages and phagocytosis of opsonized pathogens. It also plays a role in the initiation of a coagulation protease cascade and in cell migration mechanisms. The endothelial cell counter-receptor for CD11b/CD18 is ICAM-1. Monoclonal Anti-Human CD11b can be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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