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F2653 Sigma-Aldrich

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, FITC Antibodies, MO-MR,
conjugate   FITC conjugate
clone   polyclonal
biological source   goat
application(s)   flow cytometry: 1:100
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
form   buffered aqueous solution
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   secondary antibodies
grade   flow cytometry grade

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Specificity

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Immunogen

Purified Fab fragments of mouse IgG.

Application

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:
• indirect immunofluorescence staining
• immunofluorescence staining
• flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What applications have product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat,been used in?
Product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat, has been used in Flow Cytometry in the following literature references.Bergman, I. et al., Clinical Cancer Research., 7, 2050 (July 2001).Farsinejad, A. et al. Iranian Journal of Blood and Medicine, 2, Number 3, (Spring 2010).This antibody has been used in Immunofluorescencs in the following literature reference.Sushan, A., Hum. Reprod., 19 (9) : 1957-1967 (2004).
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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