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F2928 Sigma-Aldrich

Monoclonal Anti-Fas Ligand antibody produced in rat

clone 101624, purified immunoglobulin, lyophilized powder

Synonym: Anti-CD95L, Anti-FasL

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Death Receptor Ligands,
conjugate   unconjugated
clone   101624, monoclonal
biological source   rat
application(s)   neutralization: suitable
  western blot: 1-2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
Gene Information   mouse ... Fasl(14103)

Description

General description

Fas Ligand is a 40kDa type II membrane protein, which also known as FasL and CD95L, belongs to the tumor necrosis factor (TNF) family. In the new TNF superfamily nomenclature, FasL is referred as TNFSF6. It consists of TNFα, α and β chains of lymphotoxin (LT), CD40 ligand, and CD30 ligand. It also has four potential N-glycosylation sites which appear to be variably used.

Immunogen

purified recombinant mouse Fas Ligand, amino acids 132-279, expressed in NSO cells.

Application

The monoclonal Anti-Fas Ligand antibody is suitable for western blot analysis of rat thymus homogenates to detect the recombinant rFasL molecule. The antibody is also suitable for immunoblotting at a concentration of 1-2μg/ml and ELISA at a concentration of 0.5-1.0μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

FasL is predominantly expressed on activated T cells and NK cells, whereas Fas is expressed on various cell types. The gene expression is induced by the activation of mature T cells with phorbol myristic acetate (PMA) and ionomycin, concanavalin A (Con A) or anti-CD3. After cleaving of FasL, it can generate the soluble Fas ligand, a non-covalently linked homotrimer. Membrane-bound FasL and TNFα are primary activators of their receptors. Soluble FasL may inhibit the killing effect of membrane FasL. It plays an important role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. It has chemoattractant characteristics for neutrophils which indicate proinflammatory function.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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