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F3165 Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Synonym: Anti-ddddk, Anti-dykddddk

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, FK-FO,
conjugate   unconjugated
clone   M2, monoclonal
biological source   mouse
application(s)   western blot: 10 μg/mL (Protein A)
species reactivity   all
form   buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin (Purified IgG1 subclass)
isotype   IgG1
antibody product type   primary antibodies
purified by   using Protein A
concentration   3.8-4.2 mg/mL
immunogen sequence   DYKDDDDK

Description

General description

Anti Flag M2 antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F1804 is an affinity purified, FLAG M2 antibody, increasing sensitivity in most applications.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Antibody is recommended for use in immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, ELISA, electron microscopy, flow cytometry and supershift assays.

Browse additional application references in our FLAG® Literature portal.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

5 mg in poly bottle

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Inhibition of Autoregulated TFG-ß

We studied autocrine transforming growth factor (TGF)beta signaling in kidney epithelium. Cultured proximal tubule cells showed regulated signaling that was high during log-phase growth, low during c...
Keywords: Growth factors

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Peer-Reviewed Papers
15

References

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