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F3290 Millipore

FLAG® Peptide

lyophilized powder

Synonym: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

  • Empirical Formula (Hill Notation) C41H60N10O20

  • Molecular Weight 1012.97

  •  MDL number MFCD02262135

  •  PubChem Substance ID 329799698

  •  NACRES NA.32

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Properties

Related Categories FLAG Affinity Purification, FLAG System, FLAG and 3xFLAG Peptides, Molecular Biology, Plant Biotechnology,
Quality Level   200
form   lyophilized powder
mol wt   1012.97 Da
shipped in   wet ice
storage temp.   2-8°C
SMILES string   NCCCC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CC(O)=O)C(O)=O
InChI   1S/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/m0/s1
InChI key   XZWYTXMRWQJBGX-VXBMVYAYSA-N

Description

General description

The FLAG peptide is used for the competitive elution of amino-terminal, Met-amino-terminal or carboxy-terminal FLAG fusion proteins from the Anti-FLAG M1 or Anti-FLAG M2 antibody (in solution or bound to an agarose gel).

Application

Gently elutes FLAG fusion proteins from ANTI-FLAG® M1 and M2 affinity resins. Usual working concentration is 100 μg/ml. Will not elute 3X FLAG fusion proteins. For this application, use 3X FLAG Peptide, F4799.

Browse additional application references in our FLAG® Literature portal.

Preparation Note

For stock solution, dissolve in TBS (10mM Tris HCL, 150 mM NaCl, pH7.4) to a final concentration of 5 mg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Related Content

FLAG System Using three monoclonal ANTI-FLAG antibodies

The FLAG® system uses three monoclonal anti-FLAG® antibodies to detect and purify FLAG®-tag proteins. While each antibody recognizes and binds to the FLAG® epitope (corresponding to the peptide seque...
Keywords: Cell disruption, Dot blot, Enzyme-linked immunosorbent assay, Gene expression, Immunoprecipitation, Individual protein Immunoprecipitation, Ion-exchange chromatography, Precipitation, Purification, Western blot

Peer-Reviewed Papers
15

References

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