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F402-05 Sigma-Aldrich

Feline Chondrocytes: FC (Cryovial)

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Chondrocytes, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   feline articular cartilage (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 38
morphology   chondrocyte
application(s)   cell culture | mammalian: suitable
relevant disease(s)   arthritis
shipped in   dry ice
storage temp.   −196°C

Description

General description

Feline Chondrocytes, CnC, are derived from normal feline articular cartilage where they produce and maintain the extracellular matrix of cartilage.  Following monolayer culturing, FC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes that allow them to sustain normal proteoglycans production and retention.  Because of the prevalence of degenerative joint disease (DJD) in geriatric cats, FC can be a useful tool for veterinary practice.

Cell Line Origin

Cartilage

Application

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

Components

Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 1st passage, >500,000 cells in Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Subculture Routine

Please refer to the FC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles

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